In continuation to our previous work, in this article, we report on the synthesis of thiourea compounds with hexachlorocyclotriphosphazene as a hexasubstituted precursor in a typical one-pot reaction system [11]. Compounds with MIC value up to 400 ppm are considered to have inhibition activity against growth of Gram-negative bacteria, but only compounds with MIC value smaller than 220 ppm can be suggested for clinical purposes [33]. Based on the IR, 1H, 13C, and 31P NMR spectra, it was suggested that 3a–f were synthesised in one-pot reaction system and not the targeted molecule 4a–f (Scheme 1). p-chorophenyl- and p-tolyl-thiourea,”, N. B. Pappano, O. P. Centorbi, and F. H. Ferretti, “Determination of minimum concentration inhibitory chalcone derivatives,”, O. Trott and A. J. Olson, “AutoDock Vina: improving the speed and accuracy of docking with a new scoring function, efficient optimization and multithreading,”, G. M. Morris, H. Ruth, W. Lindstrom et al., “Software news and updates AutoDock4 and AutoDockTools4: automated docking with selective receptor flexibility,”, S. George, M. B. Ramzeena, S. V. Ram, S. K. Selvaraj, S. Rajan, and T. K. Ravi, “Design, docking, synthesis and anti, Y. K. Shao and J. X. Si, “Synthesis and herbicidal activity of N-(o-flourophenoxyacetyl) thiourea activities and related fused heterocyclic compound,”, K. Moriya, T. Masuda, T. Suzuki, S. Yano, and M. Kajiwara, “Liquid crystalline phase transition in hexakis (4-(n-(41-alkoxyphenyl) iminomethyl) phenoxy) cyclotriphosphazene,”, E. Cil, M. Arslan, and A. O. Gorgulu, “Synthesis and characterisationof benzyl andbenzoyl substituted oxime-phosphazees,”, G. G. Muccioli, J. Wouters, G. K. E. Scriba, W. Poppitz, J. H. Poupaert, and D. M. Lambert, “1-Benzhydryl-3-phenylurea and 1-benzhydryl-3-phenylthiourea derivatives: New templates among the CB1 cannabinoid receptor inverse agonists,”, Y.-H. Shen and D.-J. The formation of the thioureas is fast and highly chemoselective, and proceeds via formation of the intermediate isothiocyanate. The additional aromatic group in 5a-b is strongly bound to enzyme enoyl ACP reductase (FabI) of E. coli through - bond interactions (yellow colour cylindrical wireframe) with hydrophobic pockets of Phe 1251. This protocol works smoothly with aliphatic primary amines to afford various di- and trisubstituted thiourea derivatives. The synthesis of thioureas is typically accomplished by mixing an appropriate isothiocyanate with an amine at ambient temperatures, although in the literature there are some procedures that are performed at elevated temperatures (even at reflux ) in the presence of a base (e. g. Et 3 N). Review articles are excluded from this waiver policy. The chemical shifts for 31P NMR are relative to the internal standard of 85% phosphoric acid. Various thiocarbamides and oxazolidinethiones have been synthesized, including chiral thiourea catalysts and chiral oxazolidinethione auxiliaries with high selectivity. 2017, Article ID 1509129, 7 pages, 2017., 1Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia, 2Centre for Pre-University Studies, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia. Zainab Ngaini, Wan Sharifatun Handayani Wan Zulkiplee, Ainaa Nadiah Abd Halim, "One-Pot Multicomponent Synthesis of Thiourea Derivatives in Cyclotriphosphazenes Moieties", Journal of Chemistry, vol. The synthesis of the proposed hexasubstituted thioureas 4a–f was prepared via reaction of hexachlorocyclotriphosphazene with potassium thiocyanate to form isothiocyanates phosphazene intermediates, followed by typical thiourea reaction with a series of amines derivatives in a one-pot reaction system. Thiourea is widely studied and claimed to be used in many applications such as herbicides, pharmaceutical agents, pesticides, rodenticides, vulcanization accelerator, and scaffolds in organic synthesis [1]. The substitution of P-Cl bonds with various types of nucleophiles allowed the construction of phosphazenes-based ligands with different types of physical and chemical properties [16]. Potassium thiocyanate, aniline, ρ-toluidine, ρ–anisidine, glycine, L-alanine, and L-phenyl alanine were obtained from Merck and used without purification. keywords = "isothiocyanates, thioureas solid-phase synthesis, peptide coupling reagents, combinatorial chemistry". Barghash RF(1), Massi A, Dondoni A. Boas, U., Andersen, H. G., Christensen, J. The negative control was medium broth of inoculums with solvent. Various methods have been reported to make this versatile group of thiourea derivatives easily accessible with excellent yields [2, 9–12]. title = "Facile synthesis of aliphatic isothiocyanates and thioureas on solid phase using peptide coupling reagents".