• Recombinant DNA is a form of artificial DNA which is engineered through the combination or insertion of one or more DNA t d th b bi i DNADNA strands, thereby combining DNA sequences which would not normally occur together. This recombinant DNA then has to be introduced into the host. Sometimes these two terms are used synonymously. Social Impact of Recombinant DNA technology •thi iifi dhil hilhere is scientific and philosophical concern about – the use of embryonic stem cells in genethe use of embryonic stem cells in gene therapy. A recombinant DNA molecule is produced by joining together 1. one mRNA with a DNA segment 2. one mRNA with a tRNA segment 3. two mRNA molecules 4. Recombinant DNA technology (rDNA) is technology that is used to cut a known DNA sequence from one organism and introduce it into another organism thereby altering the genotype (hence the phenotype) of the recipient. However, in recent era, this field has demonstrated unique impacts in bringing advancement in human life. Such a gene is called 1. Two DNA segments 2. However, in recent era, this field has demonstrated unique impacts in bringing advancement in human life. Recombinant DNA technology in the synthesis of human insulin requires millions of copies of the bacteriawhose plasmid has been combined with the insulin gene in order to yield insulin. Recombinant DNA Technology / March 27, 2020 April 11, 2020 / By Rakesh Tools used for recombinant DNA technology are the molecules, mainly enzymes, used for the manipulation of DNA like cutting, joining, end modification etc. Eco RI digests 5' GAATTC 3'/3'CTTAAG 5' between each G and A, leaving AATT tails (overhangs) i.e. Recombinant DNA Technology Tools and Techniques R. C. Gupta M.D. Biotechnology which is synonymous with genetic engineering or recombinant DNA (rDNA) is an industrial process that uses the scientific research on DNA for practical applications. TOOLS FOR RECOMBINANT DNA TECHNOLOGY ENZYMES Restriction endonucleases (REs); Function: DNA is fragmented (digested; cut) at specific sites-found in bacteria and serve to degrade invading DNA-recognize specific base sequence that reads the same 5 to 3 on opposite strands; ex. Plasmids and bacteriophages are the most common vectors in recombinant DNA technology that are used as they have a very high copy number. Opened the doors of recombinant DNA research The most important tools of recombinant DNA technology Restriction endonucleases Discovered by Arber, Smith and Nathans in the early1970s Arber Smith Nathans 7. The insulin gene is expressed as it replicates with the B-galactosidase in the cell undergoing mitosis This process involves multiple steps that have to proceed in a specific sequence to generate the desired product. Recombinant DNA Technology- Steps, Applications and Limitations. By virtue of this technology, crucial proteins required for health problems and dietary purposes can be produced safely, affordably, and sufficiently. In the past century, the recombinant DNA technology was just an imagination that desirable characteristics can be improved in the living bodies by controlling the expressions of target genes. To use recombinant DNA technology to functionally analyze mutations introduced into cloned eukaryotic genes, a rapid procedure is necessary to assay the steps along the gene expression pathway. A recombinant DN… A gene produced for recombinant DNA technology contains a gene from one organism joined to the regulatory sequence of another gene. Concepts and Tools for Recombinant DNA Technology Byong H. Lee Invited Distinguished Professor, Department of Food Science & Biotechnology, Kangwon, National University, Chuncheon, Korea, Adjunct Professor, Department of Food Science & Agric Chemistry McGill University, Montreal, Quebec, Canada, Distinguished Professor, School of Biotechnology Jiangnan University, Wuxi, China